Monocytes
CD14+ monocytes are large agranular myeloid-derived leukocytes with roles in the inflammatory and anti-inflammatory processes of the innate immune response. They can differentiate into macrophages and dendritic cells (DC) responsible for phagocytosis, antigen presentation, and cytokine production.
The M1/M2 subtypes of macrophages are key modulators of cancer tumorigenesis. DC-based anti-tumor vaccines are also being explored for cancer immunotherapies. Monocytes are also used in regenerative medicine applications such as wound healing and tissue repair. Their role in the pathology of autoimmune diseases like lupus are providing insight to develop new treatments.
Tissue source: Leukopak
Tissue Source
Leukopaks
Isolated Cell
Monocyte cells isolated from Leukopaks are available through positive or negative immunomagnetic bead separation. Positive immunomagnetic bead separation - beads bind to the target cell population from the heterogenous mixture; labeled target cells remain while the non-target cells are removed. Negative immunomagnetic bead separation - beads bind to the non-target cell population to deplete them from the mixture leaving behind the unlabeled cells of interest.
Research & Development Applications
- Fundamental Research (proliferation, biomarker discovery)
- Drug Discovery
- Immuno-oncology
- Cell Therapy process development
At AllCells, we believe that your success is our success. We believe that sharing our knowledge and expertise is more than a business practice, it’s a responsibility. And we believe that collaboration leads to better outcomes. Below is a collection of relevant links to Monocytes resouces including blog posts, webinars and useful protocols to help you become more efficient and more effective. Have technical questions about Monocytes products? Our Customer Success Team is standing by.
WEBINARS
BLOG POSTS
- FAQ Blog Series: What’s the Difference between Positive and Negative Immunomagnetic Cell Separation?
- Why You Should Start Clinical Grade Cell Supply Discussions Early in Cell and Gene Therapy Development
- FAQ Series: The Science Behind Cryopreservation
- CD14+ Monocytes are a Critical Component of the Innate Immune System
Bulk Cell Isolation
AllCells offers bulk cell isolations of important cell types from single-donor leukapheresis and bone marrow material—standard and sequential immunomagnetic separation protocols are available. Obtain large quantities of quality-controlled cells including CD34+ HSPCs, lymphocyte populations (T cells, B cells, NK cells, and monocytes), and PBMCs for a variety of research and biomedical applications. Contact us for specific donor requests and custom isolation protocols.
Obtaining bulk quantities of highly pure and viable isolated cells from a qualified vendor offers researchers the opportunity to bank cells to support experimental consistency and comparability, and streamlines laboratory workflows.