Bulk Cell Isolations
AllCells offers bulk cell isolations of important cell types from single-donor leukapheresis and bone marrow material—standard and sequential immunomagnetic separation protocols are available. Obtain large quantities of quality-controlled cells including CD34+ HSPCs, lymphocyte populations (T cells, B cells, NK cells, and monocytes), and PBMCs for a variety of research and biomedical applications. Contact us for specific donor requests and custom isolation protocols.
Obtaining bulk quantities of highly pure and viable isolated cells from a qualified vendor offers researchers the opportunity to bank cells to support experimental consistency and comparability, and streamlines laboratory workflows.
Isolated Cell Types
CD34+ HSPCs
Hematopoietic stem and progenitor cells (HSPCs) are the key stem cell population in the bone marrow capable of self-renewal and multilineage differentiation into mature blood cell types. CD34+ HSPCs can be isolated from mobilized peripheral blood, bone marrow, and umbilical cord blood.
T Cells
T cells are the main effector cells of the adaptive immune system, characterized by the presence of a T cell receptor (TCR) on their cell surface. T cells recognize foreign antigens in the context of major histocompatibility complex molecules on antigen-presenting cells. There are three main types of T cells: cytotoxic, helper, and regulatory.
NK (Natural Killer) Cells
Natural killer (NK) cells are cytotoxic effectors of the innate immune system with a primary role in immunosurveillance. They are capable of mounting rapid immune responses against foreign pathogens and tumor cells. Primary CD56+ NK cells can be isolated from leukapheresis material (Leukopaks) via positive or negative immunomagnetic separation.
Monocytes
CD14+ monocytes are large agranular myeloid-derived leukocytes with roles in the inflammatory and anti-inflammatory processes of the innate immune response. They can differentiate into macrophages and dendritic cells (DC) responsible for phagocytosis, antigen presentation, and cytokine production.
B Cells
B cells are part of the adaptive immune system with a role in humoral immunity. B cells produce antibodies, function as antigen-presenting cells, and also secrete immunomodulatory cytokines. Primary CD19+ B cells can be isolated from leukapheresis (Leukopaks) via positive or negative immunomagnetic separation.
PBMCs (MNCs)
Peripheral blood mononuclear cells (PBMCs) consist of lymphocytes (T cells, B cells, and NK cells), monocytes, and dendritic cells, distinguished by the presence of a single round nuclei. Mononuclear cells are isolated from leukapheresis (Leukopaks) when high numbers of MNCs are required.
Save Time and Resources
Outsourcing the time-consuming process of isolating cells to an external supplier can streamline your lab’s workflow and liberate in-house personnel to focus on other priorities. Positive select and negative select isolations available.
Diverse Donor Pool
Our proprietary Donor Management System (DMS) software and dedicated team takes a holistic, targeted approach to find the right donors for any program or timeline. Gain access to collections from donors with specific attributes to meet your project needs.