High Quality Cells

Superior TNC and cell viability due to on-site processing facilities, which mitigate loss of fidelity prior to cryopreservation.


State-of-the-Art Cryopreservation

Utilizing GMP controlled-rate freezer, which enables consistently high post-thaw recovery and viability.


Dependable Cold Chain Logistics

AllCells utilizes CryoPort LN2 Dry Shippers for dependable end-to-end shipping logistics.



Cryopreserved cells give you the flexibility to do experiments on your schedule without waiting on delivery of fresh cells.

Cryopreserved Leukopaks

Leukopaks, collected from a single donor’s peripheral blood, are an enriched source of mononuclear cells (MNCs), T cells, B cells, NK cells, and monocytes. Leukapheresis collections provide higher purity and quantity of MNCs than buffy coat collection, which is desirable for research and clinical uses such as cell-based assays (proliferation, T cell activation, etc), cell therapy process development, biomarker discovery and more.

Mononuclear cells are critical raw materials for many research and biomedical applications. Freshly procured cells are viable and functional within 24 hours post-collection, however these attributes decline rapidly over time. This may cause delays and negatively impact experimental workflows, costing time, money and resources. To combat this issue, AllCells offers Cryopreserved Leukopak products, which enables researchers to have experimental flexibility without compromising on quality.

Maintain Flexibility in your Workflow without Compromising Quality

When working with fresh cells, researchers face potential delivery delays that can negatively affect their pipeline. With AllCells’ new Cryopreserved Leukopaks, you have access to high-quality primary cells whenever you need. When stored properly, Cryopreserved Leukopaks allow for prolonged storage affording you flexibility in your workflow without compromising on quality.

A key advantage with AllCells’ Cryopreserved Leukopaks is acquisition and manufacturing facilities are adjacent to each other. This enables the leukapheresis material to be immediately cryopreserved in CryoStor® CS10 (GMP cryopreservation medium) or any other cryoprotectants that customers require to maintain cell viability between collection and processing events.

MNC-rich peripheral blood is collected from healthy, consenting donors using the continuous flow Spectra Optia® Apheresis System into ACD-A anticoagulant following a standardized protocol at our on-site, IRB approved collection facilities. As with our other products, Cryopreserved Leukopaks adhere to strict quality standards to ensure unparalleled purity, viability, and quality.

State-of-the-Art Cryopreservation and Reliable Cold Chain Logistics

AllCells cryopreservation protocol has been optimized using controlled rate freezers, which utilizes state-of-the-art conduction cooling. The controlled cooling of the unit is gentle on cells, preserving cell function and viability. To ensure end-to-end cold chain logistics are worry-free, AllCells ships Cryopreserved Leukopaks using CryoPort’s LN2 dry shippers. These shippers can maintain temperatures of ≤ -150°C for up to 10 days eliminating temperature excursions and risk of product degradation. AllCells has removed the complex logistical and technical challenges, through its immediate cell processing and state-of-the-art cryopreservation protocols to safeguard product quality, providing dependable cold chain logistics to minimize risks during shipping. You can get Cryopreserved Leukopaks with high viability and functionality to achieve flexibility in your workflow without sacrificing quality.

Cryopreserved Leukopak Product Portfolio

Whether you require Cryopreserved Leukopaks with a defined starting cell number (2.5-3.0B TNC), a full collection from a single donor or have unique requirements for your research, AllCells can meet your needs. Our unique on-site collection and adjacent processing facilities can accommodate customer requests such as (but not limited to):

– A variety of aseptic division formats:

  • By cell volume or cell count
  • Have part of the collection fresh and part of it cryopreserved
  • Delivery to different geographical destinations

– Alternative cryopreservation formulations, packaging formats and more

All Cryopreserved Leukopaks are quality controlled for cell count and viability (pre-cryopreservation) using a validated AO/PI protocol. Each product meets the specifications listed below. Additional characterization such as immunopheno-typing based on cell surface marker expression (CD45+, CD3+, CD4+, CD8+, CD14+, CD56+, CD19+) and 5-part differential CBC counts will be provided.

Product LP, CR, Solo 2.5 – 3.0B LP, CR, Full Pak, Custom
Description Leukapheresis material cryopreserved in CS10 Leukapheresis material cryo-preserved per custom specification
Pre-cryopreservation TNC 2.5 – 3B Custom
Fill Volume NMT 70mL per CS250 cryo bag Custom
Sampling Vials 1+ Custom
Certificate of Analysis CBC, IP, Cell count, viability Custom

*Donor variability applies

Request a quote for your next order of high quality human primary cells.