Whole Blood

Whole human peripheral blood contains red blood cells, white blood cells, plasma, and platelets. Peripheral blood mononuclear cells (PBMCs) can be isolated from whole blood by density gradient separation to remove granulocytes and RBCs. Whole blood products are collected by venipuncture of healthy consenting donors without further enrichment in a variety of anticoagulants and formats suitable for many applications.

  • Whole Tissue
  • Isolated Cells: Mononuclear Cells (PBMCs), Eosinophils and Neutrophils, Red Blood Cells (RBCs)
  • Non-cellular Products: Plasma and Serum
AllCells can manufacture the following whole blood products:

Whole – Peripheral blood collected by venipuncture into tubes, syringes, or transfer bags. Standard anticoagulant choices are ACD:A, sodium citrate, sodium heparin, and EDTA type K2. Whole blood can also be collected into Streck DNA tubes and Paxgene DNA tubes. Please inquire if you like us to collect into other FDA approved tubes.

MNC – Mononuclear cells isolated from whole blood collected in vacutainer cell preparation tubes (CPT).

Plasma – Extracted from spinning down whole blood.

Serum – Extracted from whole blood collected in vacutainer serum separator tube (SST).

RBC – Isolated by filtration.

Buffy Coat – MNC layer collected from spinning down whole blood.

Eosinophils – Isolated by immunomagnetic microbeads.

Neutrophils – Isolated by immunomagnetic microbeads.

Whole Blood products are collected by venipuncture of healthy donors without further enrichment and thus have typical white blood cell (WBC) composition ranges. AllCells offers fresh or cryopreserved Whole Blood products collected in customer-selected or standard anticoagulants and in different tissue formats*: Whole Tissue, Mononuclear Cells (PBMCs), Subpopulations: Eosinophils and Neutrophils, Plasma and Serum.

*Cell type, cell selection method, and unit size (3-480mL) vary depending on tissue format.

Additional Search Terms: Whole blood, WB001-VK2, WB001-NH, eosinophils


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Whole Blood

Whole Blood Made-to-order

AllCells’ on-site collection and processing facilities enable us to offer custom, made-to-order (MTO) products to meet the unique needs of our customers. From tissue collection to product shipment, AllCells’ experienced team of experts will help you define, design, and deliver your customized primary cell material.

We can accommodate specific donor requests, custom whole blood isolation requirements, customer-specific anticoagulants and more.

Technical SpecificationsTech Specs
Whole Tissue

Whole Blood products are collected by venipuncture of healthy and consenting donors following IRB-approved protocols at AllCells’ onsite collection and processing facilities. Whole blood formats include vacutainer tubes, syringes, or in transfer bags in ACD-A, EDTA, heparin, or sodium citrate anticoagulants suitable for a variety of blood analysis testing.

Isolated Cells

Mononuclear cells, eosinophils, basophils and red blood cells (RBCs) isolated from whole blood collections are also available:

  • Mononuclear cells (MNCs) from whole blood are isolated by density gradient separation to remove granulocytes and red blood cells (RBCs) and washed to remove platelets.
  • Eosinophils and basophils are isolated from whole blood by negative immunomagnetic selection using beads against non-target cell populations - this leaves the isolated target cells unlabeled
  • Red blood cells are isolated from whole blood using a filtration system to remove leukocytes and platelets.

All products are quality controlled for cell count and viability determined using a validated AO/PI protocol.

Non-cellular Products:

Plasma is isolated from heparinized whole blood by centrifugation. Serum is isolated from whole blood using a serum separator tube.

Research & Development Applications
  • Fundamental Research
  • Toxicity or Immunogenicity testing
  • Genomics
  • Immuno-oncology
  • Disease modeling
Certificate of AnalysisC of A
Whole Blood CofA
Whole Blood Resources

At AllCells, we believe that your success is our success. We believe that sharing our knowledge and expertise is more than a business practice, it’s a responsibility. And we believe that collaboration leads to better outcomes. Below is a collection of relevant links to Whole Blood resouces including blog posts, webinars and useful protocols to help you become more efficient and more effective. Have technical questions about Whole Blood products? Our Customer Success Team is standing by.

Whole Blood Formats/Attributes/Unit Sizes

AllCells offers fresh and cryopreserved Whole Blood in a variety of container types and sizes. The product chart below provides detailed information on standard formats, anticoagulants and unit sizes for both cellular and non-cellular whole blood. Don’t see what you’re looking for? Our Whole Blood products can also be Made-to-order (MTO) with custom specifications to meet the specific requirements of your research project. For more information on Whole Blood MTO, please visit our MTO page or reach out to our Customer Success Team. Need immediate help? Chat with a live agent now.

Product Fresh Cryopreserved
ACD-A, Transfer Bag 162mL, 216mL, 270mL, 324mL, 378mL
ACD-A, Syringe 54mL
ACD-A, Vacutainer 8.5mL
Na Citrate, Transfer Bag 144mL, 192mL, 240mL, 288mL, 336mL
Na Citrate, Syringe 48mL
Na Citrate, Vacutainer 4.5mL
Heparin, Transfer Bag 171mL, 228mL, 285mL, 342mL, 399mL
Heparin, Syringe 57mL
Heparin, Vacutainer 10mL
EDTA, Vacutainer 10mL
SST, Vacutainer 8.5mL
PAXgene DNA, Vacutainer 8.5mL
Streck Cell-Free DNA BCT 10mL
WB MNCs nominal, custom nominal, custom
Neutrophils, NS 25M
Mature Erythrocytes (RBC) 50M
Plasma 3ml (nominal) 3ml (nominal)
4ml (nominal) Serum 4ml (nominal)

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