B cells are part of the adaptive immune system with a role in humoral immunity. B cells produce antibodies, function as antigen-presenting cells, and also secrete immunomodulatory cytokines. Primary CD19+ B cells can be isolated from leukapheresis (Leukopaks) via positive or negative immunomagnetic separation.
B cells are used in the generation of hybridomas to create therapeutic monoclonal antibodies against a target antigen. Defects in B cell development and function can cause disease so better understanding of B cell pathology in vitro can help develop new therapies and effective vaccines.
Tissue source: Leukopak
AllCells offers fresh and cryopreserved B cells derived from Leukopaks in a variety of types and sizes. The product chart below provides detailed information on standard formats, anticoagulants and unit sizes for B cells. Don’t see what you’re looking for? Our Leukopak products can also be Made-to-order (MTO) with custom specifications to meet the specific requirements of your research project. For more information, please visit our MTO page or reach out to our Customer Success Team.
|Leukopaks||CD19+ B, NS||10M||Custom, Bulk|
|Leukopaks||CD19+ B, PS||10M||Custom, Bulk|
|Leukopaks||CD19+/IgD+ Naive B, NS||1M||Custom, Bulk|
B cells isolated from Leukopaks are available through positive or negative immunomagnetic bead separation. Positive immunomagnetic bead separation - beads bind to the target cell population from the heterogenous mixture; labeled target cells remain while the non-target cells are removed. Negative immunomagnetic bead separation - beads bind to the non-target cell population to deplete them from the mixture leaving behind the unlabeled cells of interest.
At AllCells, we believe that your success is our success. We believe that sharing our knowledge and expertise is more than a business practice, it’s a responsibility. And we believe that collaboration leads to better outcomes. Below is a collection of relevant links to B Cells resouces including blog posts, webinars and useful protocols to help you become more efficient and more effective. Have technical questions about B Cells products? Our Customer Success Team is standing by.
AllCells offers bulk cell isolations of important cell types from single-donor leukapheresis and bone marrow material—standard and sequential immunomagnetic separation protocols are available. Obtain large quantities of quality-controlled cells including CD34+ HSPCs, lymphocyte populations (T cells, B cells, NK cells, and monocytes), and PBMCs for a variety of research and biomedical applications. Contact us for specific donor requests and custom isolation protocols.
Obtaining bulk quantities of highly pure and viable isolated cells from a qualified vendor offers researchers the opportunity to bank cells to support experimental consistency and comparability, and streamlines laboratory workflows.