B Cells
B cells are part of the adaptive immune system with a role in humoral immunity. B cells produce antibodies, function as antigen-presenting cells, and also secrete immunomodulatory cytokines. Primary CD19+ B cells can be isolated from leukapheresis (Leukopaks) via positive or negative immunomagnetic separation.
B cells are used in the generation of hybridomas to create therapeutic monoclonal antibodies against a target antigen. Defects in B cell development and function can cause disease so better understanding of B cell pathology in vitro can help develop new therapies and effective vaccines.
Tissue source: Leukopak
Tissue Source
Leukopaks
Isolated Cell
B cells isolated from Leukopaks are available through positive or negative immunomagnetic bead separation. Positive immunomagnetic bead separation - beads bind to the target cell population from the heterogenous mixture; labeled target cells remain while the non-target cells are removed. Negative immunomagnetic bead separation - beads bind to the non-target cell population to deplete them from the mixture leaving behind the unlabeled cells of interest.
Research & Development Applications
- Fundamental Research (proliferation, biomarker discovery)
- Drug Discovery
- Immuno-oncology
- Cell Therapy process development
At AllCells, we believe that your success is our success. We believe that sharing our knowledge and expertise is more than a business practice, it’s a responsibility. And we believe that collaboration leads to better outcomes. Below is a collection of relevant links to B Cells resouces including blog posts, webinars and useful protocols to help you become more efficient and more effective. Have technical questions about B Cells products? Our Customer Success Team is standing by.
WEBINARS
BLOG POSTS
Bulk Cell Isolation
AllCells offers bulk cell isolations of important cell types from single-donor leukapheresis and bone marrow material—standard and sequential immunomagnetic separation protocols are available. Obtain large quantities of quality-controlled cells including CD34+ HSPCs, lymphocyte populations (T cells, B cells, NK cells, and monocytes), and PBMCs for a variety of research and biomedical applications. Contact us for specific donor requests and custom isolation protocols.
Obtaining bulk quantities of highly pure and viable isolated cells from a qualified vendor offers researchers the opportunity to bank cells to support experimental consistency and comparability, and streamlines laboratory workflows.