Mobilized Leukopaks are apheresis products collected from healthy donors treated with FDA-approved drugs to stimulate the migration of CD34+ hematopoietic stem and progenitor cells (HSPCs) from the bone marrow niche into the circulation. Mobilized peripheral blood, enriched in single-donor CD34+ HSPCs is collected through leukapheresis using the Spectra Optia® Apheresis System’s continuous flow centrifugal technology. The ease of collection and high yield of single-donor CD34+ cells is a major advantage to researchers to gain experimental consistency and scalability.
AllCells is the industry leader in supplying mobilized Leukopaks with the shortest lead time on the market. We have six different mobilization regimens available using G-CSF (Neupogen®), plerixafor (Mozobil®), or a combination of the two drugs as well as CD34+ cells isolated from mobilized Leukopaks.
AllCells can manufacture the following mobilized leukopak products:
Whole – blood bag collected from leukapheresis after mobilization
(HSC) CD34+ PS – Isolated cells positive for CD34. Phenotypically characteristic hematopoietic stem cells. Isolation by magnetic microbead positive selection.
Mobilized Leukopaks are apheresis products collected from healthy donors treated with approved drugs to stimulate mobilization of CD34+ hematopoietic stem and progenitor cells (HSPCs) from the bone marrow to the peripheral blood. This procedure results in large-scale yields of single-donor CD34+ HSPCs, allowing for scalability and reproducibility of experiments by limiting the impact of donor-to-donor variability. AllCells offers Mobilized Leukopaks with different mobilization regimens, including G-CSF (Neupogen®), plerixafor (Mozobil®), or a combination of the two drugs. Mobilized Leukopak products are offered either fresh or cryopreserved, and in different tissue formats*: Whole Mobilized Apheresis Tissue, CD34 Hematopoietic Stem and Progenitor Cells (HSPCs), Multiple Mobilization Regimens Available.
*Cell type, cell selection method, and unit size (by volume or cell count) vary depending on tissue format.
Additional Search Terms: mPB026-Reg.F, mPB018F, mPB015F, mPB016F, mPB017F, mPB009, Mobilized Leukopak
AllCells' on-site collection and processing facilities enables us to offer custom, made-to-order (MTO) products to meet the unique needs of our customers. From tissue collection to product shipment, AllCells’ experienced team of experts will help you define, design, and deliver your customized primary cell material.
We can accommodate specific donor requests, custom mobilization regimens requirements, bulk isolations, cryopreservation needs and more.LEARN MORE
Whole Tissue: Healthy, consenting donors are subcutaneously injected by our qualified clinicians using the following regimen guidelines:
Filgrastim (Neupogen) 10ug/kg/day x 5 days, apheresis on day 5 and 6
Filgrastim (Neupogen) 10ug/kg/day x 5 days + Mozobil 240ug/kg/day x 2 days on day 4 and 5 (evenings), apheresis on day 5 and 6
Moz Reg A
Plerixafor (Mozobil) 240ug/kg/day x 1 day (evening), apheresis day 2 (morning); 11 hours between injection and collection
Moz Reg C
Plerixafor (Mozobil) 240ug/kg/day x 1 day (morning), apheresis day 1 (afternoon); 6 hours between injection and collection6
Moz Reg B
Plerixafor (Mozobil) 240ug/kg/day x 2 days (evenings), apheresis on day 2 and 3 (mornings); 11 hours between injection and collection
Moz Reg D
Plerixafor (Mozobil) 240ug/kg/day x 2 days (mornings), apheresis day 1 and day 2 (afternoon); 6 hours between injection and collection
Isolated Cells: CD34+ HSPCs isolated from any of the mobilized leukapheresis collections are available. Positive immunomagnetic separation is used where CD34+ beads bind to target cells in the heterogeneous population - CD34+ cells remain bound to the beads while unwanted cells are removed.
At AllCells, we believe that your success is our success. We believe that sharing our knowledge and expertise is more than a business practice, it’s a responsibility. And we believe that collaboration leads to better outcomes. Below is a collection of relevant links to Mobilized Leukopak resouces including blog posts, webinars and useful protocols to help you become more efficient and more effective. Have technical questions about Mobilized Leukopak products? Our Customer Success Team is standing by.
AllCells offers fresh and cryopreserved Mobilized Leukopaks in a variety of types and sizes. The product chart below provides detailed information on standard formats, anticoagulants and unit sizes for both cellular and non-cellular Mobilized Leukopaks Don’t see what you’re looking for? Our Mobilized Leukopak products can also be Made-to-order (MTO) with custom specifications to meet the specific requirements of your research project. For more information on Mobilized Leukopak MTO, please visit our MTO page or reach out to our Customer Success Team. Need immediate help? Chat with a live agent now.
|mLP Reg F||Neupogen x 5 days||1 Pak, 2 Pak||—|
|mLP Reg H||Neupogen x 5 days, + Mozobil x 2 days||1 Pak, 2 Pak||—|
|mLP Moz A (Mozobil)||Mozobil x 1 day (11 hrs between injection and collection, 1 collection)||1 Pak||—|
|mLP Moz B (Mozobil)||Mozobil x 2 days (11 hrs between injection and collection, 2 collections)||2 Paks||—|
|mLP Moz C (Mozobil)||Mozobil x 1 day (6 hrs between injection and collection, 1 collection)||1 Pak||—|
|mLP Moz D (Mozobil)||Mozobil x 2 days (6 hrs between injection and collection, 2 collections)||2 Paks||—|
|Reg F CD34+, PS||Neupogen x 5 days||—||0.5M, 1M, 5M, 10M, 25M|
|Reg H CD34+, PS||Neupogen x 5 days, + Mozobil x 2 days||—||1M, 5M|