Addressing Dermal Bacteria
TECHNICAL BULLETIN

Since these collections puncture the skin, the presence of dermal bacteria might be unavoidable; these types of bacteria exist on the surface of the skin and within the dermal layer. Skin preparation and draping are methods that mitigate bacterial contamination. These methods have been proven to be successful.
Risk Mitigation
According to published research, dermal bacteria can be present in bone marrow collections 1,2. These bacteria include the following species: Propionibacterium acnes, Propionibacterium humerusii, and Staphylococcus capitis. Prior to the puncturing the skin for collection, the puncture site is routinely disinfected using antiseptics. However, applying antiseptics alone does not kill all bacteria on the skin, nor does the antiseptics kill the bacteria in the deeper layers of the skin1. Therefore, a positive sterility result is not uncommon1.
Review of published literature has lead Discovery to implement additional procedures to mitigate the inherent risk of bacterial contamination during collections. In subsequent order, Discovery performs the following procedures:
- Clinicians use a betadine impregnated sterile drape over the bone marrow puncture site
- Clinicians use a two-needle method for bone marrow aspirations, wherein one needle is used per side to collect bone marrow
- Clinicians aspirate and discard ~1mL from the initial bone marrow collection site to remove any potential bacterial contaminants
Regardless of Discovery’s efforts to minimize bacterial contamination, the presence of such microorganisms are impossible to avoid altogether. Discovery will continue proactively addressing this issue by employing best practices based on empirical evidence from published research. Discovery is committed to providing the life science community with high quality human blood and bone marrow derived cells, while maintaining transparent communication with its clients. By addressing the risk associated with dermal bacteria, Discovery maintains the highest quality standards in both processing primary cells and informing its clients.
You can find additional technical resources in our Resources Section.
SOURCES
- Mollerup, Sarah, et al. “Propionibacterium Acnes: Disease-Causing Agent or Common Contaminant? Detection in Diverse Patient Samples by Next-Generation Sequencing.” Journal of Clinical Microbiology, American Society for Microbiology Journals, 1 Apr. 2016, jcm.asm.org/content/54/4/980.
- Leo AM, Salvadego MM, Piva MG, et al. “From the donor’s arm to blood product: a study on bacterial contamination of apheresis platelet concentrates.” Blood Transfus. 2007;5(3):130-3.
- Abidi, Maheen Z. et al. “ Importance of Site Preparation in Reducing the Risk of Bacterial Contamination during Bone Marrow Harvest” Biology of Blood and Marrow Transplantation, Volume 23, Issue 3, S184 – S185
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