Leukopak
Leukopaks are enriched leukapheresis products collected from healthy and consenting donors using the Spectra Optia® Apheresis System following an IRB-approved protocol. Leukopaks typically contain up to 50% T cells, 20% monocytes, 10% B cells, 10% NK cells, 3% granulocytes, and 3% hematocrit.
Leukapheresis results in high yields of single-donor mononuclear cells (MNCs) compared to venipuncture collection or buffy coat isolation. Leukopaks allow for scalability and reproducibility of experiments by limiting the impact of donor-to-donor variability. AllCells offers Leukopaks and isolated cell populations from leukapheresis material for a variety of applications.
- Whole Apheresis Tissue
- Isolated Cells: Peripheral Blood Mononuclear Cells (PBMCs), T Cells, B Cells, NK Cells, and Monocytes
AllCells can manufacture the following leukopak products:
Whole – Blood bag collected from leukapheresis (Leukopak)
MNC – Mononuclear cells isolated from leukopaks
Platelets – Platelet rich plasma from leukapheresis
(B) CD19+ NS – Isolated cells positive for CD19. Phenotypically characteristic of B cells. Isolation by magnetic microbead negative selection.
(B) CD19+ PS – Isolated cells positive for CD19. Phenotypically characteristic of B cells. Isolation by magnetic microbead positive selection.
(B) CD19+/IgD+ NS – Isolated cells positive for CD19 and IgD. Phenotypically characteristic of naive B cells. Isolation by magnetic microbead negative selection.
(CUL) Dendritic – Cultured immature dendritic cells derived from CD14+ monocytes.
(CUL) Macrophages – Cultured macrophages derived from CD14+ monocytes.
(M) CD14+ NS – Isolated cells positive for CD14. Phenotypically characteristic of monocytes. Isolation by magnetic microbead negative selection.
(M) CD14+ PS – Isolated cells positive for CD14. Phenotypically characteristic of monocytes. Isolation by magnetic microbead positive selection.
(NK) CD56+ NS – Isolated cells positive for CD56. Phenotypically characteristic of natural killer cells. Isolation by magnetic microbead negative selection.
(NK) CD56+ PS – Isolated cells positive for CD56. Phenotypically characteristic of natural killer cells. Isolation by magnetic microbead positive selection.
(T) CD3+ NS – Isolated cells positive for CD3. Phenotypically characteristic of pan T cells. Isolation by magnetic microbead negative selection.
(T) CD4+ NS – Isolated cells positive for CD4. Phenotypically characteristic of helper T cells. Isolation by magnetic microbead negative selection.
(T) CD4+/CD25+ NSPS – Isolated cells positive for CD4 and CD25 Phenotypically characteristic of regulatory T cells. Isolation by magnetic microbead negative selection followed by positive selection.
(T) CD4+/CD45RA+ NS – Isolated cells positive for CD4 and CD45RA. Phenotypically characteristic of naive T cells. Isolation by magnetic microbead negative selection.
(T) CD4+/CD45RO+ NS – Isolated cells positive for CD4 and CD45RO. Phenotypically characteristic of memory T cells. Isolation by magnetic microbead negative selection.
(T) CD8+ NS – Isolated cells positive for CD8. Phenotypically characteristic of cytotoxic T cells. Isolation by magnetic microbead negative selection.
(T) CD8+/CD45RA+ NS – Isolated cells positive for CD8 and CD45RA. Phenotypically characteristic of naive cytotoxic T cells. Isolation by magnetic microbead negative selection.
Leukopaks are collected by apheresis, a procedure in which collection and enrichment of white blood cells from multiple volumes of healthy donor peripheral blood are achieved by a simultaneous return of non-targeted blood cells to circulation. This procedure results in large yields of single-donor mononuclear cells (MNCs), allowing for scalability and reproducibility of experiments, limiting the impact of donor-to-donor variability. AllCells offers Leukopak products in fresh, cryopreserved, or frozen, and in different formats*: Whole Apheresis Tissue, Mononuclear Cells (PBMCs), Subpopulations: T Cells, B Cells, NK Cells, and Monocytes, Cultured Cells: Macrophages and Dendritic Cells.
*Cell type, cell selection method, and unit size (by volume or cell count) vary depending on format.
Additional Search Terms: Leukopak, PBMC, PB006F, PB009-2F, PB004F, PB009-1, PBMCs, PB003F, PB002, PB007, PB007-2, PB003, PB006F, Monocytes, CD14+ Monocytes, PB005f, B-cells, PB009-3F, cells
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Leukopaks Made-to-order
AllCells' on-site collection and processing facilities enables us to offer custom, made-to-order (MTO) products to meet the unique needs of our customers. From tissue collection to product shipment, AllCells’ experienced team of experts will help you define, design, and deliver your customized primary cell material.
We can accommodate specific donor requests, custom whole blood isolation requirements, customer-specific anticoagulants and more.
LEARN MOREWhole Tissue
Peripheral blood leukapheresis is collected from healthy and consenting donors using the Spectra Optia® Apheresis System collection protocol in ACD-A anticoagulant. During collection, autologous plasma is combined with apheresed cells at a 4:6 ratio. Leukopak collections result in large yields of single-donor mononuclear cells (MNCs).
Isolated Cells
We offer a variety of cell populations isolated from Leukopaks including MNCs, T cells, B cells, NK cells and monocytes.
- MNCs are isolated from Leukopaks treated with ammonium chloride to remove red blood cells (RBCs) and washed to remove platelets.
- Isolated T cells, B cells, NK cells and monocytes using positive immunomagnetic bead separation - beads bind to the target cell population from the heterogenous mixture; labeled target cells remain while the non-target cells are removed.
- Isolated T cells, B cells, NK cells and monocytes using negative immunomagnetic bead separation - beads bind to the non-target cell population to deplete them from the mixture leaving behind the unlabeled cells of interest.
All our products are quality controlled for cell count and viability and are determined using a validated AO/PI protocol.
Research & Development Applications
- Fundamental research (proliferation, T cell activation, biomarker discovery)
- Drug Discovery
- Immuno-oncology
- Cell Therapy process development
At AllCells, we believe that your success is our success. We believe that sharing our knowledge and expertise is more than a business practice, it’s a responsibility. And we believe that collaboration leads to better outcomes. Below is a collection of relevant links to Leukopak resouces including blog posts, webinars and useful protocols to help you become more efficient and more effective. Have technical questions about Leukopak products? Our Customer Success Team is standing by.
BLOG POSTS
- FAQ Blog Series: What’s the Difference between Positive and Negative Immunomagnetic Cell Separation?
- Overcoming the Global Biomaterial Supply Chain Challenge for the Development of CAR-T Therapies
- A Decade of Innovation: CAR-T Successes and Latest Developments
- Why You Should Start Clinical Grade Cell Supply Discussions Early in Cell and Gene Therapy Development
- What You Need to Know About Regenerative Medicine in 2022: Industry and Tech Trends
- A Donor-Centric Approach to Cell and Gene Therapy
